检索范围:
排序: 展示方式:
Epigenetic dysregulation in hepatocellular carcinoma: focus on polycomb group proteins
null
《医学前沿(英文)》 2013年 第7卷 第2期 页码 231-241 doi: 10.1007/s11684-013-0253-7
Hepatocellular carcinoma (HCC) development is characterized by the presence of epigenetic alterations, including promoter DNA hypermethylation and post-translational modifications of histone, which profoundly affect expression of a wide repertoire of genes critical for cancer development. Emerging data suggest that deregulation of polycomb group (PcG) proteins, which are key chromatin modifiers repressing gene transcription during developmental stage, plays a causative role in oncogenesis. PcG proteins assemble into polycomb repressive complex 1 (PRC1) and polycomb repressive complex 2 (PRC2) to impose the histone H3 lysine 27 trimethylation (H3K27me3) modification for repression. In this review, we will first recapitulate the mechanisms of two key epigenetic pathways: DNA methylation and histone modifications. Specifically, we will focus our discussion on the molecular roles of PcG proteins. Next, we will highlight recent findings on PcG proteins, their clinicopathological implication and their downstream molecular consequence in hepatocarcinogenesis. Last but not least, we will consider the therapeutic potential of targeting enhancer of zeste homolog 2 (EZH2) as a possible treatment for HCC. Improving our understanding on the roles of PcG proteins in hepatocarcinogenesis can benefit the development of epigenetic-based therapy.
关键词: liver cancer epigenetics histone modifications polycomb group proteins enhancer of zeste homolog 2 (EZH2)
Novel engineered proteins for mechanomaterials
Giuseppe Portale
《化学科学与工程前沿(英文)》 2020年 第14卷 第6期 页码 1122-1123 doi: 10.1007/s11705-020-1941-x
Paradoxical role of Id proteins in regulating tumorigenic potential of lymphoid cells
null
《医学前沿(英文)》 2018年 第12卷 第4期 页码 374-386 doi: 10.1007/s11684-018-0652-x
A family of transcription factors known as Id proteins, or inhibitor of DNA binding and differentiation, is capable of regulating cell proliferation, survival and differentiation, and is often upregulated in multiple types of tumors. Due to their ability to promote self-renewal, Id proteins have been considered as oncogenes, and potential therapeutic targets in cancer models. On the contrary, certain Id proteins are reported to act as tumor suppressors in the development of Burkitt’s lymphoma in humans, and hepatosplenic and innate-like T cell lymphomas in mice. The contexts and mechanisms by which Id proteins can serve in such contradictory roles to determine tumor outcomes are still not well understood. In this review, we explore the roles of Id proteins in lymphocyte development and tumorigenesis, particularly with respect to inhibition of their canonical DNA binding partners known as E proteins. Transcriptional regulation by E proteins, and their antagonism by Id proteins, act as gatekeepers to ensure appropriate lymphocyte development at key checkpoints. We re-examine the derailment of these regulatory mechanisms in lymphocytes that facilitate tumor development. These mechanistic insights can allow better appreciation of the context-dependent roles of Id proteins in cancers and improve considerations for therapy.
关键词: Id proteins lymphoma leukemia T cells B cells tumor suppressor oncogene
Caveolin proteins: a molecular insight into disease
null
《医学前沿(英文)》 2016年 第10卷 第4期 页码 397-404 doi: 10.1007/s11684-016-0483-6
Caveolae are a kind of specific cystic structures of lipid rafts in the cytoplasmic membrane and are rich in cholesterol and sphingolipids. In recent years, many researchers have found that both caveolins and caveolae play a role in the development of various human diseases, including coronary heart disease, hypertension, and nervous system disorders. The specific mechanisms by which caveolins induce diseases have been a topic of interest. However, a number of detailed molecular mechanisms remain poorly understood. This article focuses on the relationship between caveolin proteins and human diseases and reviews the molecular mechanisms of caveolins in disease networks.
关键词: caveolin caveolae microRNA disease signaling pathway heart tumor
A review of intelligent optimization for group scheduling problems in cellular manufacturing
《工程管理前沿(英文)》 页码 406-426 doi: 10.1007/s42524-022-0242-0
关键词: cellular manufacturing group scheduling flowshop literature review
Xiaoming LIU, Jia NING, Stephanie CLARK,
《化学科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 436-442 doi: 10.1007/s11705-009-0251-0
关键词: -lactoglobulin processing functional alternative technology environmental
《医学前沿(英文)》 doi: 10.1007/s11684-023-1022-x
关键词: acute promyelocytic leukemia plasma proteomics plasma metabolomics cross-sectional correlation network pathogenesis treatment
Emergence mechanisms of group consensus in social networks
《工程管理前沿(英文)》 doi: 10.1007/s42524-023-0277-x
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 362-372 doi: 10.15302/J-FASE-2018206
This study compared proteome profiles and morphological changes of rat jejunum in response to different dietary proteins. Fifty male Sprague-Dawley rats were fed with casein (control), and isolated beef, pork, fish and chicken proteins for 14 days. Proteome analysis, histological observation and PEPT1 quantification of the jejunum were performed. The results indicated that rats fed with chicken proteins had higher PEPT1 mRNA and protein levels (P<0.05) but lower villus height and ratio of villus height to crypt depth (V/C ratio, P<0.05) than those fed with casein and pork protein. Label-free LC-MS/MS indicated that, as compared to casein, intake of chicken protein can regulate oligopeptide transport mainly by upregulating PEPT1 protein expression and reducing dipeptidyl-peptidase activity related to biological oxidation, and can reduce oligopeptide absorption capacity by regulating Hippo signaling pathway. Although intake of beef and fish proteins had no significant effect on PEPT1 expression, they altered several signaling pathways.
关键词: Hippo signaling pathway meat protein PEPT1 proteome analysis rat jejunum
Hui Wan,Yu Xia,Jianghua Li,Zhen Kang,Jingwen Zhou
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 72-88 doi: 10.1007/s11705-016-1580-4
关键词: 2D-DIGE pqqB pyrroloquinoline quinone RNA-Seq Vitamin C
Xiaoli Song, Zhenghua Shi, Xiufen Li, Xinhua Wang, Yueping Ren
《环境科学与工程前沿(英文)》 2019年 第13卷 第2期 doi: 10.1007/s11783-019-1114-7
Maillard reaction between reducing sugars and amides happened during pretreatment. Over 90 min of TAH at the optimal condition, 67.59% sludge proteins was solubilized. 15.84% soluble proteins broke down to materials with small molecular weight.
关键词: Sludge flocs Microbial cells Hydrolysate Protein breakdown Melanoidin
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 373-381 doi: 10.15302/J-FASE-2018210
Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.
关键词: Aspergillus oryzae egg allergy egg white proteins IgE-binding ability ovomucoid
Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium
null
《医学前沿(英文)》 2016年 第10卷 第3期 页码 330-335 doi: 10.1007/s11684-016-0459-6
The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine N-methyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.
关键词: polycomb repressive complex Ezh2 expression inner ear cochlea development
《机械工程前沿(英文)》 2023年 第18卷 第1期 doi: 10.1007/s11465-022-0733-z
关键词: grinding minimum quantity lubrication carbon group nanofluid tribological mechanism
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 97-109 doi: 10.1007/s11684-016-0496-1
As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages. Isobaric tag for relative and absolute quantitation-based 2D nano-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight (MALDI-TOF/TOF) technologies were used. Global protein profiling, KEGG and PANTHER pathways, and functional categories were analyzed. Consequently, histological and tartrate-resistant acid phosphatase staining indicated the altered histological structure of condylar cartilage and increased bone remodeling activity in hard-diet group. A total of 805 differentially expressed proteins were then identified. GO analysis revealed a significant number of proteins involved in the metabolic process, cellular process, biological regulation, localization, developmental process, and response to stimulus. KEGG pathway analysis also suggested that these proteins participated in various signaling pathways, including calcium signaling pathway, gap junction, ErbB signaling pathway, and mitogen-activated protein kinase signaling pathway. Collagen types I and II were further validated by immunohistochemical staining and Western blot analysis. Taken together, the present study provides an insight into the molecular mechanism of regulating condylar growth and remodeling induced by reducing dietary loading at the protein level.
关键词: condylar cartilage mechanical loading proteomic analysis iTRAQ bioinformatics analysis
标题 作者 时间 类型 操作
Changes in structure and functional properties of whey proteins induced by high hydrostatic pressure:
Xiaoming LIU, Jia NING, Stephanie CLARK,
期刊论文
Cross-sectional network analysis of plasma proteins/metabolites correlated with pathogenesis and therapeutic
期刊论文
Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
期刊论文
Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis
Hui Wan,Yu Xia,Jianghua Li,Zhen Kang,Jingwen Zhou
期刊论文
Fate of proteins of waste activated sludge during thermal alkali pretreatment in terms of sludge protein
Xiaoli Song, Zhenghua Shi, Xiufen Li, Xinhua Wang, Yueping Ren
期刊论文
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
期刊论文
Tribological mechanism of carbon group nanofluids on grinding interface under minimum quantity lubrication
期刊论文